Physiology & Pharmacology
Soodabeh Elyasi; Jina Khayatzadeh; saeedeh zafarbalanejad; Sepideh Elyasi; mohammad ALIZADE
Volume 28, Issue 5 , November and December 2021, , Pages 675-687
Abstract
Introduction: Fibroblasts are involved in production of growth factors which are effective on cells’ growth and differentiation. They are the most adaptable cells in connective tissue with significant capacity for differentiation to the other cell group. Insulin-like growth factor-1 (IGF-1) plays ...
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Introduction: Fibroblasts are involved in production of growth factors which are effective on cells’ growth and differentiation. They are the most adaptable cells in connective tissue with significant capacity for differentiation to the other cell group. Insulin-like growth factor-1 (IGF-1) plays an important role in differentiation and growth of different cell lines. Silibinin is extracted from seeds of Silybum marianum, which it's effects in cancer cell lines, have been studied in limited studies. In this study we evaluated the silibinin effect on viability and IGF-1 gene expression in human foreskin fibroblast (HFF).Materials and Methods: The cytotoxic effect of 10, 20, 40 & 60 µM solution of silibinin was evaluated on HFF cells using MTT assay, after 24 & 48 hours. Then, the expression of IGF-1 gene was evaluated by means of real time-PCR.Results: Silibinin had toxic effect on HFF cells in dose-dependent manner after 24 hours of incubation in comparison with control group but no significant difference observed after 48 hours. Besides, after 24 hours of incubation, silibinin with a concentration of 20-40-60 µM significantly increased the IGF-1 gene expression in fibroblast cells in comparison with control group.Conclusion: Based on the results, silibinin significantly induces IGF-1 gene expression in dose dependent manner after 24 hours incubation with HFF cells. However, in cytotoxicity assay, concentration of 60 µM caused the highest rate of cell death after 24 hours. So, before introduction of this compound as a fibroblasts proliferation stimulant, more extensive studies are needed.
Haniyeh Ghorbannejad; Mohammad Alizadeh; Alireza Didarloo; Farnush Bakhshimoghaddam
Volume 27, Issue 2 , July and August 2020, , Pages 121-129
Abstract
Background: Incidences of overweight obesity are increasing in the world and it is an alarm for health community. The present study was designed to investigate the relationship between dietary habits with body mass index in Urmia. Materials and Methods: In this study, 453 female students were selected ...
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Background: Incidences of overweight obesity are increasing in the world and it is an alarm for health community. The present study was designed to investigate the relationship between dietary habits with body mass index in Urmia. Materials and Methods: In this study, 453 female students were selected by multi-stage sampling. Anthropometric indices including height, weight and waist circumference were measured using standard methods and calibrated instruments. The dietary habits were determined using a researcher-made questionnaire. Results: In this study, the frequency of side activity during meals was significantly higher in overweight and obese individuals than in subjects with normal weight (P = 0.034), and physical activity significantly higher in subjects with normal weight than overweight and obese (P = 0.001). Consumption of breakfast every day before going to school (P = 0.04), eating three meals regularly (P = 0.003), and healthy snack at school (P = 0.05) were directly related to underweight. In the multiple logistic regression model only eating three regular meals (OR = 1.837, 85% CI (1.35-2.51), P = 0.005) had a direct association with underweight. Conclusion: The results of this study show that side activity during meals increased risk of overweight and obesity. Eating all three meals regularly and eating breakfast every day before going to school were associated with an increased risk of underweight.
Haniyeh Ghorbannejad; Alireza Didarloo; Farnush Bakhshimoghaddam; Mohammad Alizadeh
Volume 27, Issue 1 , May and June 2020, , Pages 7-17
Abstract
Background: Obesity in children and adolescents and adults is one of the most serious public health concerns in the 21st Century. Meanwhile, adolescence is an important stage in creating healthy eating habits, which is usually maintained until the end of life. The present study was designed to investigate ...
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Background: Obesity in children and adolescents and adults is one of the most serious public health concerns in the 21st Century. Meanwhile, adolescence is an important stage in creating healthy eating habits, which is usually maintained until the end of life. The present study was designed to investigate the relationship between dietary patterns with body mass index in adolescents female in Urmia.
Materials and Methods: In this study, 453 female students were selected by multi-stage sampling. Anthropometric indices including height, weight and waist circumference were measured using standard methods and calibrated instruments. The usual food intake was determined using a semi-quantitative FFQ.
Results: In this study, three major dietary patterns were identified among female students. The "traditional" pattern included fruits, nuts, dried fruits, can and jams, pickles, tea and coffee, honey, vegetables, and sweets. The "high protein" pattern included legumes, organ meats, fish, egg, red meat, high-fat dairy products, cereals, and vegetables. The pattern of "fast foods and salty snacks" included the high-consumption of soft drinks, fast foods, salty snacks, sweets, sugars and juice. High protein dietary pattern [(second tertile compared to first one: (OR = 1.8, 95% CI = (1.1 - 3), P = 0.016) and third tertile compared to the first one: (OR = 1.6, (95% CI = 1.01- 2.7, P = 0.046)] were directly related to underweight. This significant association was maintained in the multiple logistic regression model (Tertile 2 vs. tertile 1: OR = 1.66, 85% CI (1.15-2.39), P = 0.048).
Conclusion: The results of this study show that a high protein diet was associated with an increased risk of underweight.
Haniyeh Ghorbannejad; Farnush Bakhshimoghaddam; Mohammad Alizadeh; Alireza Didarloo
Volume 26, Issue 6 , March and April 2020, , Pages 657-663
Abstract
Introduction: Obesity is prevalent in the Middle East and its resulted handicaps are the main nutritional and health issues. The present study was designed to investigate the relationship between insecurity with body mass index in Urmia. Methods: In this study, 453 female students were selected by multi-stage ...
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Introduction: Obesity is prevalent in the Middle East and its resulted handicaps are the main nutritional and health issues. The present study was designed to investigate the relationship between insecurity with body mass index in Urmia. Methods: In this study, 453 female students were selected by multi-stage sampling. Anthropometric indices including height, weight and waist circumference were measured using standard methods and calibrated instruments. Food insecurity was determined using a Radiometer/ Cornell questionnaire. Results: Regarding food security, there was no significant difference between obese and normal weight subjects. Food security (P = 0.04) were directly related to underweight. In the multiple logistic regression model food insecurity had inverse association with it (OR = 0.642, 85% CI (0.48 – 0.68), P = 0.033). Conclusion: The results of this study show that food security was associated with an increased risk of underweight.